Autophagy transports cytoplasmic material to lysosomes for degradation and recycling. The cargo is first engulfed into double-membraned autophagosomes that then fuse with lysosomes. Nascent autophagosomes nucleate close to the endoplasmic reticulum (ER), but the origin of the membranes is unclear. We showed using electron tomography that phagophores form membrane contacts with several organelles, including ER, ER exit sites, Golgi, endosomes and mitochondria. We currently focus on improving the preservation of our samples using high-pressure freezing and freeze substitution. Further, we use correlative light-electron microscopy to trace nascent phagophores, in order to clarify their morphology and relationships with other organelles.